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KMID : 0374019960190020193
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Ewha Medical Journal
1996 Volume.19 No. 2 p.193 ~ p.198
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A Study of in situ PCR in the Tissue Sections of Borderline Tuberculoid leprosy
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Abstract
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In situ PCR was applied to the paraffin embedded tissue sections of two borderline
tuberculoid patients which did not show acid fast bacilli on the Fite stain. In situ PCR was
performed with direct detection of Digoxigenin incorporated into PCR product. The best
condition of the direct in situ PCR was pretreatment with 0.2N HCl for 40 minutes and with
10 g/ml of proteinase K at 37℃for 5 minutes and 30 cycles of PCR. The positive signal was
observed within the cytoplasm of the most Schwann cells, epithelioid cells and a few
endothelial cells.
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KEYWORD
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